Dr. Y. W. LAM
林潤華博士
PhD (HK)
Associate Professor of Department of Chemistry
Contact Information
| Office: | YEUNG-G6619 |
|---|---|
| Phone: | +852 3442-6347 |
| Fax: | +852 3442-0522 |
| Email: | yunwlam@cityu.edu.hk |
| Web: | Personal Homepage Blog |
| ORCID ID: | 0000-0001-8929-5965 |
| Scopus Author ID: | 7202563947 |
Dr. Yun Wah Lam received his PhD training in the lab of Dr. Davina Opstelten at the University of Hong Kong. After receiving his PhD in 1997, he joined the group of Prof. Angus Lamond in Dundee, Scotland, where he developed an interest in the relationship of the architecture of mammalian cell nucleus and the regulation of gene expression. Lam uses live-cell imaging techniques and classical biochemical approaches to study protein localization and interactions in the cell nucleus. In parallel, he is involved in an international effort to map the human nucleolus proteome. Recently, in collaboration with Jens Andersen (Odense) and Matthias Mann (Munich), he adopted the technique of SILAC (Stable Isotope Labeling with Amino acids in Cell culture) to quantify, by mass spectrometry, the global dynamics of the human nucleolar proteome in response to changes in metabolic conditions. The resulting paper, having received over 100 citations in 24 months, was featured as the "ScienceWatch hot paper" in The Scientist magazine (March 2007). Lam was the recipient of the second prize of the Roche "Imagining the future" contest in 2006. He joined City University of Hong Kong in 2007.
Publications
Full pulbications can be viewed here
- DONG, H. J. & LAM, Y. W., Method of Determining Cell Cycle Stage Distribution of Cells, Patent No. US11,152,197, Priority No. 14/748,523 (2021)
- LAM, Y. W., AU, W. T. D. & SUN, H., Antimicrobial Peptide, Its Analog And Use, Patent No. US11,065,296, Priority No. 15/927,354 (2021)
- Ilyas, A. O., Alam, M. K., Musah, J., Yang, M., Lam, Y. W., Roy, V. A. L. & Lau, C., Investigation on the Direct and Bystander Effects in HeLa Cells Exposed to Very Low α-Radiation Using Electrical Impedance Measurement, In: ACS Omega. 6, 22, p. 13995-14003 (2021)
- Ilyas, A. M., Alam, M. K., Musah, J., Yang, M., Vellaisamy, A. L. R., Lam, Y. W. & Lau, C., CHO cell dysfunction due to radiation-induced bystander signals observed by real-time electrical impedance measurement, In: Biosensors and Bioelectronics. 181, 113142 (2021)
- Dong, M., Kwok, S. H., Humble, J. L., Liang, Y., Tang, S. W., Tang, K. H., Tse, M. K., Lei, J. H., Ramalingam, R., Koohi-Moghadam, M., Au, D. W. T., Sun, H. & Lam, Y. W., BING, a novel antimicrobial peptide isolated from Japanese medaka plasma, targets bacterial envelope stress response by suppressing cpxR expression, In: Scientific Reports. 11, 12219. (2021)
- Qiao, W., Wong, K. H. M., Shen, J., Wang, W., Wu, J., Li, J., Lin, Z., Chen, Z., Matinlinna, J. P., Zheng, Y., Wu, S., Liu, X., Lai, K. P., Chen, Z., Lam, Y. W., Cheung, K. M. C. & Yeung, K. W. K., 2021, TRPM7 kinase-mediated immunomodulation in macrophage plays a central role in magnesium ion-induced bone regeneration, In: Nature Communications. 12, 1, 2885. (2021)
- Tang, S. W., Tong, W. Y., Pang, S. W., Voelcker, N. H. & Lam, Y. W., Deconstructing, Replicating, and Engineering Tissue Microenvironment for Stem Cell Differentiation, In: Tissue Engineering - Part B: Reviews. 26, 6, p. 540-554 (2020)
- Lai, J. C. L., Leung, M. O. Y., Lee, D. Y. H., Lam, Y. W. & Berning, K., Biomarking Trait Resilience With Salivary Cortisol in Chinese Undergraduates, In: Frontiers in Psychology. 11, 536510. (2020)
- Shen, W., Berning, K., Tang, S. W. & Lam, Y. W., Rapid and Detergent-Free Decellularization of Cartilage, In: Tissue Engineering - Part C: Methods. 26, 4, p. 201-206 (2020)
- Xu, S., Xie, F., Tian, L., Fallah, S., Babaei, F., Manno, S. H. C., Manno III, F. A. M., Zhu, L., Wong, K. F., Liang, Y., Ramalingam, R., Sun, L., Wang, X., Plumb, R., Lee, G., Lam, Y. W. & Cheng, S. H., Estrogen accelerates heart regeneration by promoting the inflammatory response in zebrafish, In: Journal of Endocrinology. 245, 1, p. 39-51 13 p. (2020)
- Tang, S. W., Yuen, W., Kaur, I., Pang, S. W., Voelcker, N. H. & Lam, Y. W., Capturing instructive cues of tissue microenvironment by silica bioreplication, In: Acta Biomaterialia. 102, p. 114-126 (2020)
- Lam, B. P., Cheung, K. C. S., Lam, Y. W. & Pang, S. W., Two-Layer Scaffold Platforms for Drug Screening of Nasopharyngeal Epithelial Cells, 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020. Institute of Electrical and Electronics Engineers Inc., p. 412-415 9056387. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); vol. 2020-January). (2020)
- Lam, B. P., Cheung, S. K. C., Lam, Y. W. & Pang, S. W., Microenvironmental topographic cues influence migration dynamics of nasopharyngeal carcinoma cells from tumour spheroids, In: RSC Advances. 10, 48, p. 28975-28983 (2020)
- Lam, B. P., Lam, Y. W. & Pang, S. W., Using Biomimetic Scaffold Platform to Detect Growth Factor Induced Changes in Migration Dynamics of Nasopharyngeal Epithelial Cells, In: IEEE Access. 8, p. 187553-187563 (2020)
- Yeo, J. H., Lam, Y. W. & Fraser, S. T., Cellular dynamics of mammalian red blood cell production in the erythroblastic island niche, In: Biophysical Reviews. 11, 6, p. 873-894 (2019)
- Lai, J. C. L., Lee, D. Y. H., Leung, M. O. Y. & Lam, Y. W., Daily hassles, loneliness, and diurnal salivary cortisol in emerging adults, In: Hormones and Behavior. 115, 104558. (2019)
- Lam, Y. W. & Perez Bobadilla, M., Cells in search for the body: evoking the haptic memory of cultured cells, (Presented). (2019)
- Tang, S. W., Uddin, M. H., Tong, W. Y., Pasic, P., Yuen, W., Thissen, H., Lam, Y. W., Voelcker, N. H., Replication of a Tissue Microenvironment by Thermal Scanning Probe Lithography, In: ACS Applied Materials and Interfaces. 11, 21, p. 18988-18994 7 p. (2019)
- Hu, P., Berning, K., Lam, Y., Ng, I. H., Yeung, C. & Lam, M. H., Development of a Visible Light Triggerable Traceless Staudinger Ligation Reagent, In: Journal of Organic Chemistry. 83, 21, p. 12998-13010 (2018)
- Tuwahatu, C. A., Yeung, C. C., Lam, Y. W. & Roy, V. A. L., The molecularly imprinted polymer essentials: curation of anticancer, ophthalmic, and projected gene therapy drug delivery systems, In: Journal of Controlled Release. 287, p. 24-34 (2018)
- Lai, J. C. L., Leung, M. O. Y., Lee, D. Y. H., Lam, Y. W. & Berning, K., Loneliness and Diurnal Salivary Cortisol in Emerging Adults, Top 10 Contributions on Molecular Biology. Avid Science (2018)
- Lai, J. C. L., Leung, M. O. Y., Lee, D. Y. H., Lam, Y. W. & Berning, K., Loneliness and Diurnal Salivary Cortisol in Emerging Adults, In: International Journal of Molecular Sciences. 19, 7, 1944. (2018)
- Shen, W., Shen, Y., Lam, Y. W. & Chan, D., Live Imaging of Planaria, Planarian Regeneration: Methods and Protocols. Rink, J. C. (ed.). New York: Humana Press, p. 507-518 (Methods in Molecular Biology; vol. 1774). (2018)
- Dong, M., Seemann, F., Humble, J. L., Liang, Y., Peterson, D. R., Ye, R., Ren, H., Kim, H., Lee, J., Au, D. W. T. & Lam, Y. W., Modification of the plasma complement protein profile by exogenous estrogens is indicative of a compromised immune competence in marine medaka (Oryzias melastigma), In: Fish and Shellfish Immunology. 70, p. 260-269 (2017)
- Zhou, S. F., Gopalakrishnan, S., Xu, Y. H., To, S. K. Y., Wong, A. S. T., Pang, S. W. & Lam, Y. W., Substrates with patterned topography reveal metastasis of human cancer cells, In: Biomedical Materials (Bristol). 12, 5, 055001. (2017)
- Lai, J. C., Lam, Y. W., Berning, K., Leung, M. & Lee, Y. H. D., Loneliness and diurnal salivary cortisol in Chinese undergraduates, Psychoneuroendocrinology. Elsevier Ltd., Vol. 83, Supp.. p. 17-18 (2017)
- Lai, J. C., Lam, Y. W., Berning, K., Leung, M. & Lee, Y. H. D., Loneliness and Diurnal Salivary Cortisol in Chinese Undergraduates (2017)
- Xu, Y., Zhou, S., Lam, Y. W. & Pang, S. W., 17 Aug 2017, Dynamics of Natural Killer Cells Cytotoxicity in Microwell Arrays with Connecting Channels, In: Frontiers in Immunology. 8, AUG, 998. (2017)
- ZHANG, H., Xiao, P., WONG, Y. T., SHEN, W., CHHABRA, M., Peltier, R., Jiang, Y., He, Y., He, J., TAN, Y., XIE, Y., HO, D., LAM, Y. W., Sun, J. & SUN, H., Construction of an alkaline phosphatase-specific two-photon probe and its imaging application in living cells and tissues, In: Biomaterials. 140, p. 220-229 (2017)
- Dong, M., Liang, Y., Ramalingam, R., Tang, S. W., Shen, W., Ye, R., Gopalakrishnan, S., Au, D. W. T. & Lam, Y. W., Proteomic characterization of the interactions between fish serum proteins and waterborne bacteria reveals the suppression of anti-oxidative defense as a serum-mediated antimicrobial mechanism, In: Fish and Shellfish Immunology. 62, p. 96-106 (2017)
- LAI, C. L. J., LEUNG, O. Y. M., LAM, Y. W. & BERNING, K., Loneliness and Diurnal Salivary Cortisol in Chinese Undergraduates (2017)
- Zhou, Shu Fan; Gopalakrishnan, Singaram; Xu, Yuan Hao; Yang, Jie; Lam, Yun Wah; Pang, Stella W. A Unidirectional Cell Switching Gate by Engineering Grating Length and Bending Angle. PloS one. Public Library of Science. 2016;11(1):e0147801.
- Tang, QY; Qian, WX; Xu, YH; Gopalakrishnan, S; Wang, JQ; Lam, YW; Pang, SW. Control of cell migration direction by inducing cell shape asymmetry with patterned topography. Journal of Biomedical Materials Research Part A. Wiley Online Library. 2015;103(7):2383-2393.
- Poon, Ellen; Keung, Wendy; Liang, YM; Ramalingam, Rajkumar; Yan, Bin; Zhang, Shaohong; Chopra, Anant; Moore, Jennifer; Herren, Anthony; Lieu, Deborah K. Proteomic analysis of human pluripotent stem cell-derived, fetal and adult ventricular cardiomyocytes reveals pathways crucial for cardiac metabolism and maturation. Circulation: Cardiovascular Genetics. Am Heart Assoc. 2015;CIRCGENETICS. 114.000918.
- Tang, Qing Yuan; Tong, Wing Yin; Shi, Jue; Shi, Peng; Lam, Yun Wah; Pang, Stella W. Influence of engineered surface on cell directionality and motility. Biofabrication. IOP Publishing. 2014;6(1):15011.
- Liang, Yi Min; Wang, Xian; Ramalingam, Rajkumar; So, Kin Yan; Lam, Yun Wah; Li, Zhou Fang. Novel nucleolar isolation method reveals rapid response of human nucleolar proteomes to serum stimulation. Journal of proteomics. Elsevier. 2012;77:521-530.
- Li, Zhou Fang; Liang, Yi Min; Lau, Pui Ngan; Shen, Wei; Wang, Dai Kui; Cheung, Wing Tai; Xue, Chun Jason; Poon, Lit Man; Lam, Yun Wah. Dynamic localisation of mature microRNAs in Human nucleoli is influenced by exogenous genetic materials. PLoS One. Public Library of Science. 2013;8(8):e70869.
- Babaei, Fatemeh; Ramalingam, Rajkumar; Tavendale, Amy; Liang, Yimin; Yan, Leo So Kin; Ajuh, Paul; Cheng, Shuk Han; Lam, Yun Wah. Novel blood collection method allows plasma proteome analysis from single zebrafish. Journal of proteome research. ACS Publications. 2013;12(4):1580-1590.
- Cai, Xiaoning; Ramalingam, Rajkumar; San Wong, Hau; Cheng, Jinping; Ajuh, Paul; Cheng, Shuk Han; Lam Yun Wah. Characterization of carbon nanotube protein corona by using quantitative proteomics. Nanomedicine: Nanotechnology, Biology and Medicine. Elsevier. 2013;9(5):583-593.
- Tong, Wing Yin; Shen, Wei; Yeung, Connie WF; Zhao, Ying; Cheng, Shuk Han; Chu, Paul K; Chan, Danny; Chan, Godfrey CF; Cheung, Kenneth MC; Yeung, Kelvin WK. Functional replication of the tendon tissue microenvironment by a bioimprinted substrate and the support of tenocytic differentiation of mesenchymal stem cells. Biomaterials. Elsevier. 2012;33(31):7686-7698.
- Dong, Hongjuan; Shen, Wei; Cheung, Myra Ting Wai; Liang, Yimin; Cheung, Hon Yeung; Allmaier, Günter; Au, Oscar Kin-Chung; Lam, Yun Wah. Rapid detection of apoptosis in mammalian cells by using intact cell MALDI mass spectrometry. Analyst. Royal Society of Chemistry. 2011;136(24):5181-5189.
- Tong, WY; Liang, YM; Tam, Vivian; Yip, HK; Kao, YT; Cheung, KMC; Yeung, KWK; Lam, YW. Biochemical characterization of the cell-biomaterial interface by quantitative proteomics. Molecular & Cellular Proteomics. ASBMB. 2010;9(10):2089-2098.
- Lam, Yun W; Evans, Vanessa C; Heesom, Kate J; Lamond, Angus I; Matthews, David A. Proteomics analysis of the nucleolus in adenovirus-infected cells. Molecular & Cellular Proteomics. ASBMB. 2010;9(1):117-130.
Research Interests and Projects
Proteomics allow the large-scale identification of proteins in complex biological systems, but it often only produces static snapshots of proteomes that fail to describe and explain the dynamic nature of cellular behaviours. In contrast, modern cell imaging techniques allow the quantitative analysis of the spatial and temporal distribution of biomolecules in living cells, but only a relatively small number of proteins can be studied in one experiment. Our lab strives to bring together these two powerful methodologies to tackle fundamental biological questions. To this end, we perform quantitative mass spectrometry to measure the flux of a large number of proteins through subcellular spaces and to track the proteomic changes within cellular complexes through time. In parallel, we record these dynamic cellular behaviours in live cells using time-lapse fluorescence microscopy. From these two angles, we aim at building coherent pictures of intracellular events under different growth and metabolic conditions. This lab aims to apply this dual approach to a range of topics, from basic cell biology to environmental sciences.
Project lists as principal investigator
Funded by UGC Sources
- Subcellular distribution of microRNAs: Another dimension of regulation?
- Proteomic study on nuclear reprogramming during cell differentiation
- The Proteomic Landscape of the Human Nucleolus during Senescence
Funded by Non-UGC Sources
- Teaching development grant:"Using programmed learning to help applied biology students to prepare for laboratory classes"(Completed)
- New staff startup grant: “A novel approach for studying mammalian nuclear import using SILAC-based proteomics"(Completed)